Tissue selective insulin
concentrations in adipose tissue


aOFM was successfully used to measure insulin concentrations and time-resolved profiles in a clinical trial


 

 

Challenge

Insulin serum concentrations might not reflect actual concentration of insulins at the tissue level. Insulins designed to act in the liver benefit from the direct measurement of absolute insulin tissue concentrations to assess the expected PK and dynamic concentration profiles for several days on tissue level.

Solution

In a euglycaemic glucose clamp study aOFM was used to sample ISF from adipose tissue. Insulin concentrations were continuously measured for 36 h in 24 type 1 diabetes patients and absolute concentrations were determined via a no-net-flux approach.

Results

aOFM was successfully used to measure insulin concentrations and to obtain time-resolved profiles in a clinical trial.

The ISF-to-serum concentration ratios for the new tested insulin were only half relative to human  insulin. Absolute concentration and dynamic concentration profiles in serum were significantly different from adipose tissue.

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